I’m looking for a program that can quantity the difference between images in an image sequence over time.
We are hoping to use time lapse images to measure the activity of tadpoles by comparing how the images change over time. Tracking the movement of individuals isn’t necessary. The tadpoles are dark and the background of the aquarium is light, however the background isn’t uniform and some of the decor items like dark rocks and foliage make it so that the all tadpoles aren’t visible at all times.
I’m probably doing a terrible job of explaining this, so let me know if you need me to clarify anything. Any and all suggestions appreciated!